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The 2004 Annual Meeting (January 14-20, 2004) of OASYS_NEW |
Not yet assigned to a slot - 12:24 AM
Drug Delivery System of bFGF for Peripheral Nerve Regeneration
Takamatsu K1, Kaneshiro Y1, Nakatsuka H2, Koshimune M3, Enomoto M1, and Takaoka K1. (1) Orthop. Surg, Osaka City University, 1-4-3, Asahimachi, Abeno-ku, Osaka, Japan, (2) Dept. of Orhop. Surg, Seikeika Hosp, 4-2-10, kouryounakamachi, Sakai, Japan, (3) Dept. of Orthop. Surg, Saiseikai Nakatsu Hosp, 2-10-39, shibata, kita-ku, Osaka, Japan
Recently, various biodegrable polymer tubes have been reported. However those results were not satisfying enough for clinical use. The purpose of this study is to introduce our biodegradable polymer tube as drug delivery system (DDS) and to report its effect on peripheral nerve regeneration and angiogenesis. We prepared our tubes in 125I bFGF solution for 10 min. After that we implanted them into subcutaneous tissue of Wistar rat and measured the residual 125 bFGF at 1h, 1D, 7D and 14D post implantation. we have implanted these tubes with or without bFGF into 12 mm sciatic nerve gaps in Wistar rats. At 12 weeks after implantation, number of regenerating nerves and vessels were observed histologically. 80% bFGF could remain at 1h after implantation, 60% at 1D, 40% at 7D, and 20% at 14D. After all 40% bFGF had been gradually released during 2 weeks. concerning bFGF, the results of nerve regeneration at 12 weeks obviously showed the advantage of DDS of bFGF. On the other hand, the number of vessels and total area of vessels significantly increased in tube with bFGF. we believe in that our tube is a reliable drug delivery system and it is very useful for tissue engineering methods in peripheral nerve repair. And this DDS could have a significant effect on peripheral nerve regeneration.