The relative importance of preserving the innervation to the lower eyelid during facial reconstruction or blepharoplasty continues to be hotly debated. There is little doubt that preserving the nerves to the lower eyelid will help to prevent the morbidity of scleral show, ectropion, and dry eyes following these procedures. Conventional wisdom has suggested that lateral innervation to the orbicularis oculi muscle is the most clinically important. This study attempted to identify the general nerve pattern to the lower eyelid in humans and utilized histomorphometric analysis to determine if a dominant motor nerve branch to the lower eyelid exists. The nerve supply to ten orbicularis oculi muscles were mapped out in a fresh cadaver model and then harvested for histologic analysis. All motor nerves to the lower eyelid were identified using an operative microscope and marked B1, B2, B3, and B4, lateral to medial, from the insertion of the lateral canthus to the medial canthus, respectively. On average four branches were identified in each specimen for analysis, located between 0.3 and 3.5cm from the lateral canthus along the inferior border of the muscle. Total number of nerve fibers and density (number of fibers/mm²) for B1, B2, B3, and B4 were 269 and 4601, 188 and 3648, 332 and 4130, and 197 and 4578, respectively. The histomorphometric analysis demonstrated no significant difference in the motor nerve branches, but the branches medial to the lateral limbus of the eye (B3 and B4) contributed equally to motor unit support to the lower eyelid. The preliminary results of this study suggest that there is no single dominant nerve branch to the orbicularis oculi muscle of the lower eyelid and, therefore, preservation of the lateral nerve branches during surgery on the lower eyelid is no more important than preservation of the more medial branches.