Purpose of the study: Metabolic intermediate Fructose 1,6 diphosphate (FDP) has been seen to bypass the effect of ischemia-reperfusion injury in affected tissue. Prior administration of FDP to ischemic-islanded pedicled skin flaps increases the area of survival significantly. The purpose of this study was to evaluate whether intravascular administration of FDP helps in salvaging ischemic microvascular rat muscle tissue transfer. Method and Materials: 48 adult male Sprague Dawley rats (400-450 gm) had their right Gracilis muscle flap harvested and subsequently intra-arterially irrigated with FDP (0.125 g/kg body weight) The flap was then anastomosed to left femoral vessels after varying periods of ischemia. The ischemia time was set at 1,1.5,2,2.5, 3 and 3.5 hours (n=4 in each group). Equal no. of rats were not given FDP treatment to serve as controls. The muscle flaps were re-examined at 72 hours for viability by visual confirmation and histological examination. Frozen sections were taken at the same time for semi-quantitative estimation of surviving muscle using electrophoretic estimation of DNA fragmentation of tissues as well as oxidating mitochondria by Succinate dehydrogenase NitroBlueTetrazolium(NBT) stain. Results: Although visualization and histological examination did not show any statistically significant difference in survival of the flaps as related to period of ischemia, the FDP treated flaps showed decreased inflammatory damage in surviving flaps at the 1-1.5 hour ischemia period. This was further confirmed by better quantification of DNA fragmentation electrophoretically in FDP treated flaps as well as better staining of surviving oxidative mitochondria by Succinate NBT staining method. Conclusion: Administration of FDP after flap harvest did not affect flap survival relative to ischemia time. FDP did, however, decrease inflammatory damage in surviving flap after ischemia time of 1-1.5 hours. Such an effect could be useful in salvage of complicated flaps or replanted tissue requiring re-exploration or multiple anastomotic revision. Further methods of delivery are being investigated to obtain better survival rates.